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This is supported by our data, as these two genes have a moderate evolutionary rate but low level of functional constraint (high d N / d S rate ratio).
The data collection and filtering produced a 2.8 Mbp long dataset composed of 3012 alignments of inferred transcripts from genes for 21 mammalian and one avian species with a moderate evolutionary rate.
However, analyses performed with only characters carrying a moderate evolutionary rate or with the CAT + mtArt, which has been confirmed as an effective model to overcome the effects of Long Branch Attraction (LBA) [ 68], consistently resolved a monophyletic Branchiopoda clade.
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Instead pinniped evolution is characterized initially by low to moderate evolutionary rates.
The results of the present analysis of a large number of species including on the one hand representatives of all of the main lineages of the Characiformes and on the other a large dataset including genes with slow to moderate evolutionary rates provided insight into the phylogenetic relationships of a number of previously problematic taxa.
Accordingly, following Sperling et al. (2009), we explored the distribution of signal in the alignment by separating the sites with moderate evolutionary rates (i.e., those most likely to represent the most reliable source of phylogenetic signal) from the slowly and rapidly evolving sites (those more likely to convey misleading signal).
On the other hand, a CAT tree based on the sites with moderate evolutionary rates (fig. 7 B ) supports monophyly of commonly accepted Pancrustacea, Chelicerata, and Arachnida groups rendered poly- or paraphyletic in the analyses of heterogenous sites (fig. 7 A ).
First, when the Class III proteins are compared with the rest of the proteome, the main effect of expression level changes sign, with higher expression levels associated with a moderate increase in evolutionary rate.
These analyses produced moderate increments in the estimated evolutionary rate for nd5 as more partitions were considered, in agreement with the results from other mtDNA genes, supporting the reliability of these estimates.
A simple comparison of mean evolutionary rate recovers a moderate but statistically significant reduction in rate in essential genes, as has previously been reported (mean Poisson distance in essential genes = 0.160, nonessential = 0.174, P < 10−15, Mann–Whitney U test); (Koonin 2005; Wolf et al. 2010).
We sought to determine whether long intergenic ncRNAs (lincRNAs) follow the same evolutionary trend and indeed detected a moderate but statistically significant negative correlation between the evolutionary rate and expression level of human and mouse lincRNA genes.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com