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Most of the target compounds showed minimal anti-proliferative activity (IC50 > 37 μM), indicating they would have moderate cytotoxicity when administered as chemical absorption modifiers.
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In the current study, when the original organisms (not hybrids) were tested, S. polydactyla exhibited moderate cytotoxicity (IC50; 20 μg/ml, SI; 0.34) in HeLa cells.
Compounds 483 485 exhibited moderate cytotoxicity inhibition with hFXR transactivation (IC50 3.9, 1.5, 2.5 µM).
Additionally, compounds (189, 5) showed a moderate cytotoxicity against HEK293 cell line (IC50 7.63, 1.48 µM).
Virenamide A (237) exhibited effective cytotoxicity against various cultured cells with P388 (IC50 2.5 µg/mL), and showed moderate cytotoxicity against A549, HT29, and CV1 cells line (IC50 10 µg/mL).
Compounds 370, 371 exhibited moderate cytotoxicity against P388 cells with IC50 4.1 and 5.5 ng/mL and the other compounds showed poor cytotoxicity compared to control one.
The designed compounds demonstrated low to moderate cytotoxicity in several cell lines.
Most of the 18 prepared compounds showed moderate cytotoxicity against several human cancer cell lines.
Rohitukine showed moderate cytotoxicity against human HL-60 promyelocytic leukemia and HCT-116 colon cancer cells [56].
The metabolite (298-300) showed moderate cytotoxicity against Ehrlich carcinoma cells (EC50 values of 61, 35, 97 µg/mL), respectively.
Compounds (333 335) showed moderate cytotoxicity against MDA-MB-231 breast cancer cells (IC50 1172.53 74.41, 74.41 µM), respectively.
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