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To answer, we conducted a longitudinal histological study examining consequences of a moderate contusion at different time points postinjury.
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We first tested this hypothesis in a moderate contusion as defined by Basso et al. [3].
We applied BBB scale in days after the lesion to rats with moderate contusion in spinal cord at thoracic level.
The high number of macrophages we measured at 8 weeks in all groups was similar to the value that is generally assessed in the acute phase of SCI [53], but others authors have showed in rats the presence of a second peak in the macrophage/microglial response at 60 days after moderate contusion [55].
All rats were fully paralyzed at day 0 and 1 day after the moderate contusion injury and the rats showed no observable movement or slight movement of one or two joints (BBB, 0 1).
Adult rats received a T10 moderate contusion.
A moderate contusion injury was delivered using 150 kdynes of force with a 1 s dwell.
A few HL training studies have been performed in a moderate contusion injury which results in moderate functional deficits e.g., [29] [30].
Following a moderate contusion the HLs can stand and also perform partial weight-bearing steps [30]; whereas following severe contusion the HLs are practically paralysed and have no ability to generate any weight-supporting steps [26].
We have previously shown locomotor recovery in immunodeficient mice that received a moderate contusion spinal cord injury (SCI) and hCNS-SCns transplantation 9 days post-injury (dpi).
Moderate contusion SCI was produced using a NYU-MASCIS injury device as previously described [ 25, 26].
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