These comprised: negative (<1% carcinoma cells stained), borderline (1 5% cells stained), intermediate (5 25% cells stained), moderate (25 50% cells stained), strong (50 75% cells stained) and very strong (75 100% cells stained).
We performed a semi-quantitative scoring of TAMs by measuring the density of CD68+, CD163+ and also of myeloid CD11b+ cells as follows:(1) no, or sporadic cells; (2) moderate numbers of cells; (3) abundant occurrence of cells; and (4) highly abundant occurrence of cells.
TIL scoring of tumours was performed semiquantitatively by measuring the densities of CD3+, CD8+, CD4+ and FOXP3+ cells as previously described (Dahlin et al, 2011): (1) no, or sporadic cells; (2) moderate numbers of cells; (3) abundant occurrence of cells; and (4) highly abundant occurrence of cells.
Under these conditions, AdTRAIL caused moderate cell killing in H460 cells with a viability of 78.6±6.9% of control noninfected cells.
As shown in Figure 5, mice immunized with the BCR/ABL-pIRES-hIL-2 (d) caused moderate cell inflammatory cell infiltration, and those immunized with BCR/ABL-pIRES (c) and pIRES-hIL-2 (e) induced mild cell infiltration.
As shown in Figure 5A, low dose of gemcitabine (1.0 μg ml−1) resulted in BxPC3 cells in only moderate cell death.
Slides were evaluated by two independent observers and scored on a scale of 0 3: 0, absent positive tumor cells; 1, weak cell staining or <10% positive cells; 2, moderate cell staining or 10 50% positive cells; and 3, intense cell staining or >50% positive cells.
Importantly, although siRNA was able to completely knockdown the expression of HKII in both cell lines, such a knockdown caused only moderate cell death in MCF7 cells while significantly more apoptotic cells were observed in MDA436 cells.
Recently, a series of pyrrolidine-based compounds with good selectivity for nNOS in cell-free assays and moderate cell permeability have been reported (Xue et al., 2010a, b).
Human umbilical vein endothelial cells and B16.F10 melanoma cells showed only moderate reductions in cell viability of about 50 and 30%, respectively.
The weighted score is obtained by multiplying the staining intensity score (3+, strong positive stain in most cells; 2+, moderate stain in cells; 1+, weak stain in cells; 0, no evidence of stain) and score for the percentage of positive cells (3+, most of cells stained; 2+, half of cells stained; 1+, few cells stained; 0, no cells stained).
