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Crucially, the assembly was created using free or open-source bioinformatics tools, on commodity computing hardware (16 cores; 64Gb RAM) using only a moderate amount of data.
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However, this is not always the case, and robust species trees can sometimes be recovered from moderate amounts of data.
With N = 100 and only moderate amounts of data (S = 50; L = 10), most estimates clustered around 100 and only 0.3% were higher than 500.
When effective size is moderately small (Ne = N = 100), good precision can be obtained even with moderate amounts of data [CV < 0.2 for S = 50, L = 10].
Crucially, the finished genome was achieved using only moderate amounts of data and assembled on commodity computing hardware, suggesting that high-quality, finished bacterial genomes can be achieved at very low cost with only a small amount of bioinformatics infrastructure.
Overall, our simulations suggest that, although the percentage of nodes supported is always a decreasing function of additional data analyzed, relatively high overall support values can probably be obtained with moderate amounts of data.
Fortunately, because the LD signals for large and small populations are quite different (Fig. 3), estimates based on even moderate amounts of data should be able to provide a useful lower bound for Ne, and this can be important, particularly in conservation applications where a major concern is avoidance and/or early detection of population bottlenecks.
The results of our experiments demonstrate this new fire module can simulate a wide range of fire regimes across heterogeneous landscapes with a few parameters and a moderate amount of input data.
However, the review was limited by the moderate amount of available data on several cause specific mortality outcomes.
Last, the performance evaluation was limited in that we examined a single site with a moderate amount of missing data, and measurement noise, especially important at the low CO levels encountered, was not considered.
This situation is not unusual, as the number of organisms where there is even moderate amounts of sequence data are severely limited.
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