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Induced mutagenesis combined with phenotyping tools offer significant opportunities for linking gene models with putative functions.
In the P. berghei ANKA genome, we identified 73 gene models with putative protein kinase domains (Table S1).
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This procedure predicted a total of 6,029 protein-coding gene models with 1,063 putative spliceosomal introns in 947 of them.
The EST clusters and transcripts determined by Cufflinks were used to update the gene models with extra putative exons, mainly at the 5′ and 3′ end of genes.
In contrast, docking generated relatively few models with the putative interface for eIF2Bδ2, eIF2Bαβ, and eIF2Bαδ, placing the highest-scoring models at least 25 Å interface rmsd away in each case, confirming that these combinations do not form dimers.
For structural proof and to obtain information required for eventual molecular modelling associated with putative biological targets, crystalline samples of the DQ amide 16 and the quinoline carbamate 22 were sought for X-ray structure determination.
To account for this mechanism, we updated the model with a putative CaMK-dependent RyR leak (see Methods section for details), which produced a ≈ 3-fold increase in the resting Ca2+ leak for CaMK3X vs. WT (data not shown).
The AIC values were compared for models with and without putative outliers (process two) and the best model included 17 samples (indicating RudP, Ash, Gata, RudB and RudC were true outliers; Table 4).
But our results do show that the selective pressure as measured by codon models is not related with putative adaptive evolution of AARs.
Our results are compatible with putative models of the (SSB 35 structure (Raghunathan et al., 2000) and reveal a likely wrapping configuration for the (SSB 56 mode.
Our EC annotation of the JGI v4.0 transcript models identified 1,427 predicted transcripts with putative enzymatic functions.
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