Sentence examples for models were cultured from inspiring English sources
Both culture models were cultured in DMEM with 10% foetal bovine serum, 100 IU/mL penicillin, 100 μg/mL streptomycin, and 1 mM glutamine and maintained in the same incubator (5% CO2, at 95% humidity) for the same times, and the medium was refreshed twice a week.
The tissue models were cultured at the air-liquid interface in 0.7 mL media in cell culture inserts (24-well format).
Models were cultured for 10 days at the air-liquid interface (ALI) and imaged using an upright Zeiss LSM 510 Meta confocal microscope.
After 7 days of cultivation, three-dimensional cultured skin models were cultured at 37°C for 2 hours in Alamar Blue-containing medium.
NativeSkin® models were cultured in 12-well plates in a proprietary and chemically-defined hydrocortisone- and serum-free medium supplemented with 100 μg/mL penicillin and 100 μg/mL streptomycin in a humidified atmosphere of 5%% CO2 at 37 °C.
Furthermore, when histamine-exposed skin models were cultured for a prolonged period of time (i.e., up to 11 days instead of 7 days), epidermal differentiation remained impaired, indicating that histamine did not merely cause a delay, but rather an arrest of keratinocyte differentiation (Fig. S4).
This three-dimensional (3D) tissue model was cultured initially submerged, then raised to the air/liquid interface for up to 28 days.
No invasion was observed when the normal mammary duct model (MCF10a-lined lumen) is cocultured with fibroblasts or when the DCIS lumen model is cultured alone.
The histamine-induced changes in epidermal differentiation are still observed in organotypic skin models that were cultured for longer time period.
NIE 115 mouse neuroblastoma cells chosen here as a neuronal model [32] were cultured in a complete growth medium containing 90% Dulbecco's modified Eagle's medium with 4.5 g/L glucose (supplemented with sodium pyruvate) and 10% fetal bovine serum.
In this model, human chondrocytes were cultured in alginate beads and stimulated with soluble mediators secreted from NDSF and RASF, respectively.
