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Since our cell models were cultivated in the presence of E2 and our data showed that 17β-HSD1 positively regulates ERα mRNA level, one could postulate that the positive correlation between nm23-H1 and 17β-HSD1 expressisn is coincident with the activation of nm23-H1 expression by ERα and E2, which are increased with 17β-HSD1 expression.
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This is largely because of the fact that most of the OIS cellular models are cultivated in glucose excess.
The tissue of our in vitro model was cultivated on membrane inserts at the interface between air and culture medium to ensure adequate in vitro nutrition and oxygen supply (Metzger et al. 2007).
In our slice model cholinergic neurons were cultivated for two weeks with NGF from beginning resulting in around 120 detectable ChAT+ neurons.
To study the ability of PLA nanoparticles to transport flurbiprofen across an in vitro BBB model, bEnd.3 cells were cultivated in the luminal compartment of cell culture inserts until a post-confluent monolayer had grown.
They were cultivated and westernised.
In some cases, vegetables were cultivated.
Only small plots of wheat were cultivated.
The cells were cultivated overnight.
Cells were cultivated in Plus MDCK.
All yeasts were cultivated at 30°C.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com