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To examine Usp9x tumorigenicity and consequence of Usp9x inhibition in human pancreatic tumor models, we carried out gain- and loss-of-function studies using established human pancreatic tumor cell lines (PANC1 and MIAPACA2) and four spontaneously immortalized human pancreatic patient-derived tumor (PDX) cell lines.
Thus, in our models we carried forward values from prior questionnaires.
Having identified the best-fit clock and demographic models, we carried out a suitable number of independent MCMC runs to achieve convergence both with effective sample size estimates that usually exceeded 200 (usually after 100 million generations, visualised with Tracer v1.5).
To test whether the results were robust to alternative models, we carried out sensitivity analyses accounting for patients with multiple admissions (by including index hospital admissions only) and hospitals that closed or opened during the study period (defined as a hospital contributing no patients to the dataset during the first or last period).
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What I came to realize is that it's a much more complex set of emotions around the mental models we carry around in our heads, essentially," he said.
In order to statistically assess the good specification of a model, we carried out an analysis of residuals.
To evaluate the efficiency of the L-CG-MEC model, we carried out experiments using IBM CPLEX solver [30].
In order to validate the performance of the proposed model, we carried out a number of simulations and experiments.
By using the proposed model, we carried out several numerical simulations on fracture process in different specimens.
Using the inverse model, we carried out experiments to control dynamic responses of electroadhesion force, which successfully controlled force responses against pulse voltages.
Based upon an approximate NAND flash memory device model, we carried out simulations and the results clearly demonstrate the potential effectiveness of the proposed design solution.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com