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For LPS-mediated endotoxemia models, mice were injected i.p. with the designated dose of LPS (E.coli O127 B8, Sigma-Aldrich) dissolved in PBS containing 0.25% bovine serum albumin (BSA).

In addition, to study the physiological responses in animal models, mice were exposed to 10% oxygen for 3 days.

AML models, mice were implanted with 5 × 10 cells (or 1 × 10 for MV4 11 cells) in the right flank.

At the end of the cardiac-injection experiments (21 days for 1833-BM1 and 13 days for the 4T1 models), mice were anesthetized and immobilized with tape in the imaging tube of a Skyscan 1178 μCT.

For evaluation of the percentage of apoptotic cells in treated and untreated nude mice models, mice were sacrificed by cervical dislocation after scanning, and tumor issues were collected and performed with TUNEL assay to determine DNA damage.

For recovery of EF3030 and mutants from mice in the pneumonia and colonization models, mice were killed 7 days postinfection, samples were collected, and CFUs were counted in nasal washes, lungs, and blood as described previously (19,20).

Despite being poor pathogenesis (natural disease) models, mice are used extensively in evaluation of vaccine immunogenicity and protective efficacy.

We set a humane endpoint in the tumour model; mice were sacrificed when the tumour volume reached 2000, mm3, which has been approved by the Animal Ethics Committee of Keio University.

For cytokine studies in the sepsis model, mice were sacrificed by decapitation 12 h and 24 h after bacterial inoculation to harvest blood.

In the C57BL/6-to-BALB/c model, mice were lethally irradiated and then rescued with an allogeneic donor BM. 2 × 106 GMSCs or fibroblast cells were injected the same day as acute GVHD was induced.

The model mice were orally treated with DHA (10 mg/kg) at 12 days after birth and their bilirubin levels were measured at 14 days after birth, 48 hours after the treatment.