In animal models, gene targeting in induced pluripotent stem cells has successfully been deployed to correct the Z-antitrypsin defect and to restore the ability of the mammalian liver to secrete active antitrypsin.
Based on important metabolites indicated by the model, gene targets for increasing growth rate under 1-butanol stress were determined, and new strains with higher growth rates were predicted and validated.
Those users cover a range of disease research from QTLs to strain models to gene targets.
As a result, researchers have been trying to elucidate the function using a combination of cell biological and biochemical methods and the construction of animal models using gene targeting in mice.
As they generated more oncomice and knockout mice, the mouse models with individual gene target provided even more information when crossed with mice with various genetic backgrounds.
To address whether haploinsufficiency of TNNT2 is partially or completely responsible for either HCM or DCM, we ablated Tnnt2 in a mouse model by gene targeting.
They utilized a Poisson mixture model to identify gene targets with differential Pol II binding activities in breast cancer MCF7 cell line under various conditions.
A popular model postulates that gene targeting to the NPC in yeast is not based on an active transport but relies on the continuous movement of genes within the nucleus and their attachment to the pore when they reach the nuclear periphery by passive diffusion (Dion and Gasser 2013).
Our MuRF1 and MuRF2 KO mouse models correspond both to constitutive null models (for details on gene targeting, see Supplementary Figure 1B D).
Meyer, M., Ortiz, O., Hrabé de Angelis, M., Wurst, W. & Kühn, R. Modeling disease mutations by gene targeting in one-cell mouse embryos.
