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The fate of PPi at the active site has become a major focus of attention in models for translocation of polymerases along a template.
In contrast, sequential, 'binding change' models for translocation receive extensive support.
Several models for translocation through the NPC have been proposed [ 30- 33], although the lack of a direct way to study the movement of protein through the NPC hinders an accurate and quantitative characterization of the translocation mechanisms.
The roles of the pulmonary macrophage in airway and alveolar clearance are described, and the applicability of recent solubility models for translocation or deposited materials to liver, skeleton, or other systemic organs is discussed.
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Our results support a model for translocation of VgrG C-terminal effector domains into target cell cytosol by a process that requires trafficking of bacterial cells into an endocytic compartment where translocation is triggered by an unknown signal.
In this case it is not entirely clear how this fits into the model for translocation of proteins into the ER.
The calculation of kon and koff is based on the mathematical formulation of a three-state model for translocation and nucleotide triphosphate binding, in which r1, r2, kon, and koff are determined simultaneously from the data.
We used dwell time samples extracted from ionic current traces and a two-state kinetic model to determine the translocation rates for complexes formed with DNA1-H_H; we used a three-state model for translocation and primer strand transfer between the polymerase and exonuclease sites to determine the translocation rates and primer strand transfer rates for complexes formed with DNA1-OH_H.
Using comparative Y chromosome information, we were able to look for common TEs in the region possibly responsible for the translocation of the SDR, identify a candidate element, and propose a model for translocation of the SDR cassette throughout salmonid genomes.
Our data are consistent with structural models for dsDNA translocation and support the proposal that dsDNA and ssDNA translocases share a similar gross mechanism for motion along different polynucleotide tracks (Hopfner and Michaelis, 2007).
Previous models for DNA translocation into T. thermophilus have placed TtPilF with other proteins associated with TFP biogenesis [ 13]: in particular, by analogy with work conducted on TFP assembly in other organisms, we would expect TtPilF to function alongside other inner membrane biogenesis components, such as PilC, PilM, PilN and PilO, to promote pilus formation.
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