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Centroids for the five largest sub-clusters were then taken as the five input models for refinement.
Many 3D maps are reconstructed for the variance analysis; the most representative ones are then used as initial models for refinement.
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As Engadget explains, the company delayed the 10.1-inch model for refinement, but as it was approaching launch, the screen manufacturer discounted the Wikipad's panel.
These 3D points are subsequently fitted to an articulated hand model for refinement of the first estimation.
This was then used as an initial model for refinement of the UDP-GlcNAc analogue complex.
A starting model for refinement (R18; PDB ID: 4E3T) (Tokuriki et al., 2012) was used to provide initial phases.
We used the reconstruction obtained from the more populated RCT class as the initial model for refinement against single particles of the full 0° data set.
Approximately 20 30 projection averages of unique and different classes representing different particle orientations were selected and used to generate an initial three-dimensional model for refinement.
3D reconstructions were generated using the Relion 1.3 3D Classification procedure with a low resolution ellipsoid as a starting model for refinement using C3 symmetry.
(E ) Comparison between the initial negative stain EM map (Snider et al., 2006 ), the cryoET sub-tomogram average map used as an initial model for refinement in this study and the final 11 Å cryoEM map.
The initial models used for refinement had alanine at the substituted sites and no fluoro alcohol, and the electron density maps showed no density for methyl groups for valine residues at position 203 or 207; however, there was clear density for the alcohols.
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