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Surprisingly, these models for nucleotide substitutions in overlapping genes were rarely cited, not to mention used, by the majority of studies estimating selection in overlapping genes.
As an integrated framework of ClustalW, PhyML, MODELTEST, ProtTest, and several in-house programs, PALM evaluates the fitness of 56 substitution models for nucleotide sequences and 112 substitution models for protein sequences with scores in various criteria.
PALM is an integrated framework of ClustalW, PhyML, MODELTEST, ProtTest and several in-house programs to evaluate the fitness of 56 substitution models for nucleotide sequences and 112 substitution models for protein sequences with scores in various criteria.
Prior to the B/MCMC, the models for nucleotide substitutions were selected using the hierarchical likelihood ratio test (hLRT) implemented in the Modeltest 3.7 program.
The models for nucleotide substitutions were selected for each gene individually, prior to the MCMC, and using the Akaike Information Criterion (AIC [ 48]).
The models for nucleotide substitution used in the analyses were selected for each gene individually by applying the Akaike Information Criterion (AIC, Akaike [ 30]) using the program MrModeltest 2.2 [ 31] in conjunction with PAUP* [ 32].
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We used the HKY model for nucleotide sequence analysis and the JTT model for amino-acid sequences analysis.
Distance matrixes were generated using the Tamura Nei model for nucleotide and PAM model for amino acid using the MEGA 4.0 program [13].
PhastCons uses genome alignments of 17 species and a continuous-time Markov model for nucleotide substitutions and a two state phylo-HMM model to compute posterior conservation probabilities.
The DNADIST program of the PHYLIP package, using the Jukes-Cantor model for nucleotide substitution was used to generate the distance matrix analyzed by LIBSHUFF.
With the fishing cat as outgroup, we constructed phylogenetic trees using the software PAUP* (version 4.0) [25] and the best-fit model for nucleotide frequencies, transition-transversion ratio and nucleotide substitution by the Akaike Information Criterion (AIC) [26] in ModelTest (version 3.8) [28], [28].
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