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Instead, users must collate models from each of the specific OMIM Parkinson disease records and models annotated in structured text in order to create a complete list.
We defined integration as having landed in a gene only if it was within the genomic coordinates of the 21,322 protein-coding genes or transient EST gene models annotated in the zebrafish genome.
Eight of the 15 gene models annotated in the database required extensive changes, which were performed by visual inspection of DNA sequencing traces, comparative amino acid sequence alignments and after cDNA verification by RT-PCR.
However, some gene models annotated in this 12X draft were found to contain greater frequencies of intron-exon splices not supported by EST evidence (data not shown) and, therefore, the 12X draft was not used.
These clusters were identified by using CD-hit (Li and Godzik 2006) to cluster gene models annotated in the de novo assemblies of all 48 resequenced strains with 70% amino acid identity.
The total of 20,482 gene models annotated in X. maculatus, resulted in transfer of 20,300 and 20,325 gene models over to X. couchianus and X. hellerii, respectively.
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NTF is the total number of TFs annotated in A. thaliana and is the number of TFs that the model suggests to be perturbed.
An additional search strategy was to use the word " Hsp20" as a keyword to identify gene models annotated as Hsp20 in the soybean genome, which could potentially be missed when only the HMM profile is used due to the presence of incomplete domains.
The number of annotated genes in this high confidence set was considerably higher than the number of gene models annotated with a GO term in P. pruinosa and P. euphratica where 11,587 [ 40] and 9296 in [ 9] were reported respectively, possibly reflecting improved annotations tools.
We identified this set by retrieving the complete set of protein-coding models in Zebra finch from Ensembl BioMart (ensembl.org/biomart/martview), and excluding all models annotated as "Known", models with orthologs in other species, and models placed onto 'chromosome Unknown', which are thought to largely represent allelic variants.
In rice, a total of 66,433 gene models were annotated in the MSU rice genome annotation database (http://rice.plantbiology.msu.edu/; Ouyang et al. 2007; Kawahara et al. 2013), from which 40,800 non-transposable element (non-TE) and 14,278 TE probes (60 bp each) were designed, and the remaining genes were not suitable for probe design due to the sequence homology of other genes.
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