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The log-likelihoods, substitution models, and tree topologies were compared among independent runs using SH tests.
The Modeltest-derived models and tree scores from maximum likelihood, Bayesian and parsimony analyses for all phylogenetic datasets appear in Table 3.
Parameters of the models and tree branches were estimated by maximum likelihood prior to the sampling.
This tree is also consistent across different substitution models and tree inference methods (NJ, ML or parsimony), all of which yielded the same tree (data not shown).
However, both the traditional and the alternate tree topologies can be supported by the data depending on which evolutionary models and tree construction methods are selected.
This method is independent of a priori defined demographic models and tree reconstructions, and is thus suitable for taxa with complicated population history.
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The results of this analysis are robust across the different codon substitution models and trees.
To avoid systematic error leading to inconsistent topologies due to long branches, out-groups were removed for this test and models and trees were recalculated.
The nonparametric bootstrapping step was employed in order to generate a large number of models and trees that were similar to the seven models and trees inferred from the empirical data, and yet incorporated a degree of variation as might be observed if one were to sample additional similar loci.
Evolutionary distances were based on the Kimura 2p model and tree reconstruction on the neighbor-joining method.
The default settings for window size, a step size, replicates used, gap stripping, distance model, and tree model were, respectively, 200, 20, 100, "on", "Kimura", and "Neighbor Joining".
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