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(C ) Demonstration of RNA pol II structural recapitulation from a random starting configuration (top panel) of individual pol II subunits represented as rigid bead models and restrained by cross-links (green links) and electron microscopy (EM) density map (mesh).
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Many cycles of model building and restrained refinement with tight geometric restraints were carried using overall temperature factor and NCS (non-crystallographic symmetry) restraints.
As mentioned above, several schemes have been proposed to model and restrain virus propagation, but these schemes may have some weaknesses with regard to the current prevalent virus.
Using the same model and restraining the analysis to those with a last participation in the race in either of the last two years of the inclusion period (1997 1998), provided a similar estimate (hazard ratio 1.10 per race, 95% CI 1.04 1.16).
The model was completed in alternating cycles of model correction in COOT102 and restrained refinement in Refmac5103.
Extensive model building was performed manually with COOT, and restrained refinement was performed using REFMAC5.
The animal model was then placed in an imaging chamber and restrained by mild compression to a thickness of 15 mm.
Model building was performed with COOT [ 23] and restrained refinement with REFMAC in CCP4 [ 21] and PHENIX [ 24].
The model includes the effects of cross-section asymmetry, shear deformation, rotational inertia and restrained warping.
Simple and restrained.
Ando's work is refined and restrained.
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