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A sequence alignment of E. coli and T. aquaticus Pol III used for the modelling was calculated with Clustal (Larkin et al, 2007) using Pol III sequences from 35 different bacterial species.
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Therefore, another regression model was calculated (see Table 9).
An ANOVA model was calculated for every combination of measurement.
A multi-model average (final model) was calculated across all models with delta AIC < 2.
Docked conformations of all inhibitors were superimposed and a pharmacophore model was calculated with LIQUID.
The substitution model was calculated from data for ML while BLOSUM was used for NJ.
The additive genetic model was calculated using Armitage's trend test.
Pairwise sequence divergence using the K2P-substitution model was calculated in MEGA v 4.0.
When heterogeneity was present, a random effects model was calculated.
Both an un-adjusted and adjusted model was calculated.
The discriminative ability of both new models was calculated.
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