Among them is the estimation of growth parameters which provide the basis for biological modeling and site-specific management.
The interaction of 5-formyltetrahydrofolate analogs with murine methenyltetrahydrofolate synthetase (MTHFS) was investigated using steady-state kinetics, molecular modeling, and site-directed mutagenesis.
Furthermore, a homology modeling and site-directed mutagenesis studies predicted the active site architecture, which suggested the crucial active site residues for the observed substrate preference.
Making use of available data from crystallographic analyses, homology modeling and site-directed mutagenesis, the present review focuses on assessment of the topology of prospective key players dictating allosterism.
Constitutively active mutants (CAMs) of AT1 receptor have been engineered using molecular modeling and site-directed mutagenesis approaches among which substitution of Asn111 in the transmembrane helix III with glycine or serine results in the highest basal activity of the receptor.
In order to determine, if such an arrangement is conserved in YqeH too, a combination of homology modeling and site-directed mutagenesis was employed.
Combining molecular modeling and site-directed mutagenesis, it appears that PDCD10 is a six helical bundle protein composed of a two heptad repeat, and α5 is an important amphipathic helix housing five lysine residues essential for PtdIns 3,4,5 P3 binding.
Protein sequence alignment, structure modeling and site-directed mutagenesis demonstrated that Ser, Gly, Asn, Asp and His residues composed the active centre of 'AcTesA.
