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Our aim was to determine whether ballooned hepatocytes have potentially disabled the cell death execution machinery, and if so, can their functional biology be modeled in vitro.
Animal models are pivotal for studies of pathogenesis and treatment of disorders of the central nervous system which in its complexity cannot yet be modeled in vitro or using computer simulations.
These effects can be seen in vivo as well as modeled in vitro.
Chronic, low dose, in vivo effects of any reagent are often appropriately modeled in vitro, by proportionately higher doses of that same reagent, over more acute time frames.
These early stages of satellite cell activation can be modeled in vitro by culturing isolated myofibres in medium containing horse serum and chick embryo extract.
To test this hypothesis, the hypertrophic response was modeled in vitro by treating cultured NCM's with PE as above, and levels of myostatin precursor protein and activated myostatin were analyzed.
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LMNA-related DCM was modeled in-vitro using patient-specific iPSC-CMs.
It has been demonstrated in [ 23, 26, 27] that the enzymatic activity of RISC can be efficiently modeled in-vitro as a classic Michaelis-Menten reaction, where the target mRNA is the substrate, the siRNA-loaded RISC is the active enzyme (at a constant concentration), and the product is the degraded mRNA.
An interesting feature of muscle fiber cellularization is that it can be modelled in vitro using skeletal muscle myotubes [20].
It is difficult to model (in vitro) partially healed fractures or the contribution of soft tissues to stability accurately.
By such integration, scientists can model In vitro assays using In silico descriptor packages while making benefit of multi-learning features and automatics applicability domain estimation.
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