For example, Barnett et al. [25] performed adenoviral intra-arterial gene delivery of anti-angiogenic endostatin in a syngeneic rat gliosarcoma model and demonstrated transgene expression and therapeutic efficacy.
Perhaps most valuable to the field of breast cancer is the widely used mouse mammary tumour virus (MMTV -driven PyV MMTV -drivenel, in which transgene exPyVssion occurs in the mTmouse epitheliumodel].
However, as in other large animal models, rAAV-mediated transgene expression declined over time.
In addition, SCNT using pools of stable transfected cell clones was an efficient way for the production of transgenic founder pigs with appropriate expression patterns (reviewed in [ 1, 9]) and facilitated the generation of the first pig models with inducible transgene expression [ 10].
To better understand the role of PAX3 in differentiated melanocytes, we have generated a transgenic mouse model in which PAX3 transgene expression is under the control of tyrosine promoter.
This is in contrast to most other transgenic models of SCC, where transgene expression is targeted to the basal proliferative layer (e.g., oncogenic Ras, [56]).
Thus, amplicons can be used not only for molecular farming purposes, but they can also serve as model systems for analyzing transgene expression and virus infection processes, as well as antiviral defense mechanisms.
Therefore, it serves as a useful tool for assessment of gene delivery and also a model of choice for investigating transgene expression for future lung gene therapy.
Utilization of novel in vitro models of neurodegenerative diseases with transgene expression by nucleofection, aiming to facilitate drug discovery.
Bergoglio and colleagues reported that tumor induction could not be seen in a Pol β Tg mouse model with thymus-specific Pol β transgene expression, suggesting that Pol β over-expression is not sufficient to initiate tumorigenesis in vivo [59].
