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Using bioprinting, we generated three-dimensional (3D) intestinal tissue composed of human primary intestinal epithelial cells and myofibroblasts with architecture and function to model the native intestine.
To model the native ascites tumor environment as closely as possible in vitro, we analyzed bulk ascites cell pellets, which in addition to CD8+ and CD4+ T cells, contained resident tumor cells, Tregs, B cells, NK cells, and CD14+ cells (Table 2).
4) Rosetta was used to model the native complex based on the 3-way cross-linker.
However, more complicated diseases will require culture methods that more closely model the native tissue environment.
Although DP can accurately predict a minimum energy structure within a given thermodynamic model, the native fold is often in a suboptimal energy state that significantly varies from the predicted one [ 19].
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Besides, most models in the largest cluster are near the lowest-energy model, therefore the native model.
Firstly, these techniques can be applied to system-level designs by using suitable abstraction techniques to extract finite state models from the native models or applying them to parts of the system that are intrinsically finite state.
The basic protein model used was a minimalist Gō model using the native structure to determine attractive energies in the protein chain.
The structure was solved by molecular replacement in PHASER, using the crystallographic model of the native Pol α as search model, and refined to 3.1 Å resolution with BUSTER and PHENIX, to R-factor/R-free of 21.1/24.8.
Two models were used simultaneously for the calculations; the first model was the native TmPurL structure (PDB entry 1VK3); the second search model was that of PurLQS constructed based on the StPurL structure (PDB entry 1T3T).
Compared to the niche model of the native clade in Europe, the projected model for America suspected European Carychium to inhabit regions at lower and higher latitude (see Additional file 2C).
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