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In this model the flap is harvested around a cutaneous perforator arterial branch by twisting of the vascular pedicle and rotating the skin paddle like a propeller up to a maximum angle of 180°.
In support of this model, the flap endonuclease FEN1 limits CAG·CTG repeat expansion in several systems (Spiro et al. 1999; Liu et al. 2004, 2009; Yang and Freudenreich 2007; Goula et al. 2009).
The processing of a 5′ flap was previously proposed as a step in meiotic recombination (Osman et al. 2003), although in this model, the flap was generated during resolution of a recombination intermediate into a crossover rather than as a step in producing a conversion event unassociated with a crossover.
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Accurately reproducing the dynamics of Oyster requires the introduction of a new reference mathematical model, the "flap-type absorber".
Disregarding the folded flap hanging off the top left side of the model, look at the main body of the model, including the flap hanging off the right.
The model for the flap consists of two rigid bodies, one fixed and the other rotating, with a single actuator connected to each body.
Push the triangle from one model under the flaps in the middle of the other model.
The practical use of a nonlinear optimisation algorithm with a FE bridge aeroelastic model, which includes the flap dynamics, necessitates the use of reduced-order models.
In this work, our theoretical results indicate that, to perform the 5'-nuclease activity, it is reasonable that the 5'-nuclease domain transits from its equilibrium position to the position near the polymerase active site (the flexible PolI model) rather than the flap DNA substrate transits from the polymerase active site to the 5'-nuclease active site (the rigid PolI model).
A simple extension of the model of flap processing to allow the interconversion of 5′ and 3′ flaps would enable exonucleases of opposite polarities to control expansion frequency as shown in Figure 6.
In the flap-processing model, the preference for expansion when CAG repeats are on the lagging-stand template can be explained by the presence of the more stably folded CTG repeat sequence on the newly synthesized lagging strand that is required to fold back on itself to form the expansion precursor.
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