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We show here that MD simulations of model systems provide a molecular-level rationale for this observation.
Fluorescence microscopy studies using giant unilamellar vesicles (GUVs) as membrane model systems provide a unique methodology to quantify protein binding, interaction, and lipid solubilization in artificial bilayers.
Model systems provide useful tools to assess the effects of established compounds in different clinical situations.
Model systems provide more favorable conditions for observing phenomena and testing hypotheses than other systems afford.
Although assays based on cell lines are useful, whole animal model systems provide much more relevant information on the activity and possible side effects of novel compounds.
Moreover, studies in other model systems provide direct evidence that the TGF-β pathway can suppress the Wnt/β-catenin pathway [ 35- 37].
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These model systems provided direct support for the "protein-only" hypothesis and indicated that fungal prions correspond to self-propagating amyloid or amyloid-like assemblies [9] [13].
It necessitates the development of new model systems providing parameters predictive for NM action in various disorders and pathophysiological conditions.
Hence, prediction of lncRNAs' functions with multiple model systems provides guideline for further experimental investigations [ 7, 8].
As described below, we found that isradipine was neuroprotective in all four of our model systems, providing an important 'proof of principle' that this combination of progressively more complex bioassays can be used to identify neuroprotective drugs that mitigate the deleterious effects of neurotoxic amyloid.
Therefore, we believe that the methylation events identified in the in vitro model system provide potential markers for cervical cancer detection as well, which is underlined by their large overlap with methylated gene promoters found in cervical carcinomas.
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