While our in vivo model system permits direct cell-cell contact between retinal microglia and RPE cells, the in vitro system used here brings the two cell types in close proximity but however precludes direct cellular surface contact.
This model system permits the exposure of respiratory epithelium to injurious agents in an easily visualized and controlled environment.
We plan to verify our findings in a mouse progression model system, permitting the elimination of the confounding and data compromising effects due to patient variability and treatment.
The properties of this model system permit the direct visualization and analysis of each stage of mammary tumorigenesis from normal mammary tissue in the uninduced state to hyperplasias, atypical hyperplasias, invasive carcinomas, and distant metastases that arise as a consequence of oncogene activation.
Few other model systems permit simultaneous identification of slow-growing CSCs and progenitor cells.
In model systems permitting genome-wide genetic interactions assays, all genes are either knocked down or knocked out and these perturbations can therefore affect the studied trait, for example fitness, by increasing it or decreasing it.
Nonetheless, these model systems permit a detailed study of the inflammatory milieu and support molecular probes of inflammatory signaling that are not possible in inflamed human synovium or primary human cells.
Developing a cardiac model system would permit examination of the relationship between the expression level of a potentially protective foreign gene and the degree of protection from injury.
By analyzing the viscoelastic properties of two distinct layers, a layer of "soft" vesicles and a "rigid" bilayer, we have created a model system to permit the study of film behavior in the region of nonlinear mass and frequency change (non-Sauerbrey).
In conclusion, we have described an in vitro model system that permits isolation of CM that transiently proliferate in monolayer cultures in vitro.
