These results, and those of our previous study with maltose binding protein model system, confirm that non-optimal codons are important for expression and export of secretory proteins via both the SecB-dependent and -independent pathways.
Taken together, this new model system confirms the potential impact of toxic CUG repeat-containing mRNA on early developmental processes and provides a valuable new tool in the armamentarium of research on myotonic dystrophy.
The low unspecific background of this new in vitro model system was confirmed by phase contrast microscopy and RICM.
In conclusion, using fission yeast as a model system we confirm that moderate levels of stress due to oxidative metabolism during the logarithmic growth may prepare cells to encounter future periods of starvation or inactivity, and that a MAP kinase pathway has an essential role in linking endogenous stress and the activation of a genetic fitness program.
Recently, several studies in hydrogel model systems have confirmed that matrix stiffness, both initially and over time, does have a significant effect on fibroblast activation within in vitro cell culture, as will be discussed further (Section D).
Note that the oligoimides were specifically used as model systems to confirm our expectation of improved chemical compatibility between the BAPS-MMT and the polyimide system.
In general, using the human GPCR model system, our results confirmed that the position of a gene in the gene networks has great influences on the likelihood of expansion of its gene family in evolution.
Thus, binding of Fg to M5 inhibits complement deposition in the mouse system, confirming that the mouse model is suitable for studies of Fg-binding to M5.
The recognition of biologically relevant pathways in gene expression profiles derived from amplified RNA in the model system tested here confirms for the first time that the amplification of bacterial RNA may be used successfully in bacterial transcriptomics.
Successful in vivo application of the CRISPR/Cas system confirms its importance as a genetic engineering tool for creating animal models of human diseases and its potential use in gene therapy.
