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The ability of Skp to prevent the aggregation of model substrates in vitro is independent of ATP.
[18F] FS-PTAD was conjugated to model substrates in moderate to good RCY under mild aqueous conditions (phosphate buffer, pH 7), however, basic conditions (sodium hydroxide, pH 9 10) were required to conjugate [18F] FS-PTAD to tyrosine.
These assays measure the correlation between increased enzymatic activities towards model substrates in resistant mosquitoes and are a general indicator of the enzyme families putatively involved.
Protein QC pathways have classically been elucidated using mutant model substrates in yeast (Jung et al. 1999; McClellan et al. 2005; Wolf and Schafer 2005).
Using linkage-specific assembly systems, we generated Lys63-, Lys48-, Lys11- and Met1-polyubiquitinated model substrates in vitro, (see Experimental Procedures), which were treated with a panel of DUBs.
We first explored the proposed acylarylation reaction using benzoic acid 1 a and N-methyl N-phenylmethacrylamide 2 a as the model substrates in the presence of the photocatalyst, DMDC, and 2,6-lutidine under visible-light irradiation (Table 1).
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The fermentation products formed during the acidogenic fermentation of maize silage as a model substrate in a leach-bed process were determined by gas and liquid chromatography.
Two independent batch fermentations were carried out over 21 d with the synthetic model substrate in the presence of the trace metal solution.
For these reasons, we selected ash as a model substrate in this experiment.
It was previously shown that degradation of the VHL model substrate in yeast requires SSE1 (McClellan et al. 2005).
Acetate is often used as model substrate in MEC systems because it is an end product of fermentation.
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