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Self-assembled monolayers (SAMs) of alkanethiols on gold are highly controllable model substrates and have been employed to mimic the extracellular matrix for cell-related studies.
Photocatalytic studies were performed using two selected substrates, Methyl Orange and Phenol, both as single model substrates and in mixtures of them.
In order to elucidate substrate-induced changes in cellulosome composition, we grew C. thermocellum on pretreated switchgrass and several other model substrates and analyzed the changes in subunit profile of cellulosomes isolated from cell-free broth using quantitative proteomics (15N metabolic labeling and LC-MS/MS).
(E ) Schematic representation of the model substrates and their amino acid sequences.
Here, we characterize the main T. reesei cellulases along with N188BG in terms of inhibition by IOS using both low Mw model substrates and C-labeled cellulose substrates.
Solid lines represent linear regression, dashed lines represent an optimal theoretical correlation with identical effects of the loss-of-function allele on both the model substrates and drug.
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Preheating whole model, substrate and particle shows a coordinated deformation pattern, penetration way and spreading way, respectively.
Two polyamide 6,6 substrates with different constructions, namely a model substrate and a fabric, were hydrolyzed using native cutinase and L182A cutinase mutant (from Fusarium solani pisi) and a protease (subtilisin from Bacillus sp).
Five subclones was found to have significant activity using a fluorescent cellulose model substrate, and three of these were observed to be highly thermostable.
For example, in his original work on the small-world model, Watts [39] explored the dynamics of Kuramoto oscillators on a WS model substrate, and showed that the fraction of synchronised oscillators had a phase transition that occurred for progressively smaller p as the oscillators' symmetric coupling strength increased (for fixed n, K).
DTT-denatured bovine αLactalbumin (Sigma L6010) was used as a model substrate and light scattering at 360 nm was used to monitor protein aggregation in the presence and absence of WT- and mutant HSPB5 at 42°C.
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