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Five subclones was found to have significant activity using a fluorescent cellulose model substrate, and three of these were observed to be highly thermostable.
Preheating whole model, substrate and particle shows a coordinated deformation pattern, penetration way and spreading way, respectively.
Two polyamide 6,6 substrates with different constructions, namely a model substrate and a fabric, were hydrolyzed using native cutinase and L182A cutinase mutant (from Fusarium solani pisi) and a protease (subtilisin from Bacillus sp).
For example, in his original work on the small-world model, Watts [39] explored the dynamics of Kuramoto oscillators on a WS model substrate, and showed that the fraction of synchronised oscillators had a phase transition that occurred for progressively smaller p as the oscillators' symmetric coupling strength increased (for fixed n, K).
DTT-denatured bovine αLactalbumin (Sigma L6010) was used as a model substrate and light scattering at 360 nm was used to monitor protein aggregation in the presence and absence of WT- and mutant HSPB5 at 42°C.
Enzymatic activities of TrxR1 and its mutants were determined by using 5,5′-dithiobis-2-nitrobenzoic acid (DTNB) as a model substrate, and the formation of TNB− was monitored as the increase in absorbance at 412 nm (ɛTNB, 412 nm=13 600 M−1 cm−1).
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Self-assembled monolayers (SAMs) of alkanethiols on gold are highly controllable model substrates and have been employed to mimic the extracellular matrix for cell-related studies.
Photocatalytic studies were performed using two selected substrates, Methyl Orange and Phenol, both as single model substrates and in mixtures of them.
In order to elucidate substrate-induced changes in cellulosome composition, we grew C. thermocellum on pretreated switchgrass and several other model substrates and analyzed the changes in subunit profile of cellulosomes isolated from cell-free broth using quantitative proteomics (15N metabolic labeling and LC-MS/MS).
(E ) Schematic representation of the model substrates and their amino acid sequences.
45 2,4,6-Trimethoxybenzoic acid (1 a) and N-cyanopiperidine (2 a) were chosen as model substrates and the catalyst and ligand loadings were set at 2 and 3 %, respectively.
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