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In accordance with the results obtained in the urethane model, subsequent analysis of Ki67 and cleaved caspase 3 staining demonstrated that both sunitinib and DC101 treatments significantly reduced proliferation and increased apoptosis in ADC tumor cells (Fig 3).
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The alignment was analyzed in ProtTest v. 2.3 to determine the most appropriate protein model for subsequent analysis [20].
The GLM procedure was used to identify significant fixed effects to be included in the model for subsequent analysis.
The bully/victim categorization interaction effect with country was found not to be significant (χ[3]= 6.3, p =.098) and was thus dropped from the model The subsequent analysis yielded significant main effects for the bully/victim categorization, gender and country (see Table 4).
Because the same set of control variables was included in different models, we will not repeat the same model in the subsequent analysis.
The model and our subsequent analysis revealed a potential quiescent ILC state, as well as a novel functional spectrum of ILCs, validated in vivo, ranging from quiescence to a classical ILC2 state to a pathological, mixed ILC3-like state.
Several steps were taken in the model development and data analysis to mitigate the potential impact of the unbalanced (in terms of gender) sample in the model results and subsequent analysis.
For reasons of parsimony, we used the one-factor model for the subsequent analysis.
However, to avoid any additional ambiguity due to structural rearrangements, we used Rab5 as the GTPase model in our subsequent analysis.
CLA performed all molecular and in vitro cellular assays, cell line characterization, in vivo animal model work and subsequent analysis of tumors by FACS, immunohistochemistry and morphometric analysis in addition to writing this manuscript.
The signal was amplified (Grass P-5), band pass filtered (100 Hz – 1 kHz) and led to an oscilloscope (Tektronix 5111), audio monitor (Grass AM 8) and FM tape recorder (Vetter Model D) for subsequent analysis.
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