The efficiency of protein separation by the developed CA membranes have been quantified using model proteins such as pepsin, egg albumin (EA) and bovine serum albumin (BSA).
Furthermore, model proteins such as trypsin (20 kDa), pepsin (35 kDa), egg albumin (45 kDa) and bovine serum albumin (69 kDa) rejection experiments were carried out under identical operational conditions employing both the PPSU and blend membranes.
The model proteins such as trypsin (20 kDa), pepsin (35 kDa), egg albumin (45 kDa) and bovine serum albumin (69 kDa) rejection experiments were carried out under identical operational conditions employing both PPSU and blend membranes.
Several model proteins such as bovine serum albumin (BSA), hemoglobin (Hb), and chymotrypsinogen (ChTg) were tested at pH values ranging from 2 to 12 to determine the role of the charge state of the protein on protein detection by a weak polyelectrolyte such as PDMAEMA.
Besides proteins from pathogens, model proteins antigens such as ovalbumin (OVA), β-galactase (β-gal) and bovine/human serum albumin (BSA/HSA) are often used in the vaccine formulation development.
Moreover, selection of more compatible model proteins without such stringent requirements could lead to more encouraging results.
Finally, it will be interesting to integrate into our model additional proteins such as the sterol transfer protein OSBP.
There are already some crystal structures of pMHC complexes available, and there are many softwares and databases allowing reliable homology modelling of proteins, such as Modeller (6), ModBase (7), HHPred (8), Phyre (9), I-tasser (10) and so forth.
Widely used substitution models for proteins, such as the Jones-Taylor-Thornton (JTT) or Whelan and Goldman (WAG) models, are based on empirical amino acid interchange matrices estimated from databases of protein alignments that incorporate the average amino acid frequencies of the data set under examination (e.g JTT + F).
Studies of intranasal delivery of model protein antigens such as recombinant influenza nucleoprotein (rNP), β-Gal and OVA together with the adjuvant c-di-AMP/IMP in mice suggest them as potent mucosal adjuvants, especially when cellular immunity is desired (Ebensen et al., 2007; Libanova et al., 2010; Ebensen et al., 2011; Sanchez et al., 2014).
Given the coordinates of the model protein, parameters such as the distribution of phi, psi and chi torsion angles and additional parameters including proline phi angles, peptide bond planarities, disulfide bond lengths can be measured.
