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A model protein of cytochrome c (CytC) was exemplified to be accommodated in the resultant MSNs and its loading amount was correlated with their pore size.
Photoactive yellow protein (PYP), which is a model protein of photoreceptor and is a prototype of PAS domain superfamily, has the unfolded N-terminal domain in its signaling state.
Interestingly, these two celullar processes are closely interconnected since I kB-alfa, the key modulator of NF- kB, can be regarded as a model protein of regulation of signal transduction by the ubiquitin-proteasome system (UPS).
To construct feedback control elements, we inserted an RNA motif (K-turn, K-loop, or a nonfunctional RNA motif) upstream of an open reading frame encoding the L7Ae protein (or one of its variants) fused to a model protein of interest, enhanced cyan fluorescent protein (L7Ae-ECFP).
From the time its structure was first analyzed (Blake et al., 1962, 1965), lysozyme has been a well-studied protein and the model protein of choice for many new methods in crystallography (Boutet et al., 2012; Cipriani et al., 2012; Nederlof et al., 2013).
Fig. 3 presents in a different form the relationships shown in Fig. 2a at log = 0. Note that the same proteins predominate at the extremes of oxygen fugacity represented in Fig. 3 and in Fig. 2a (reducing – early Golgi; oxidizing – vacuole) and that the reference model protein of microtubule appears with low relative abundance.
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To demonstrate the model and theory, three model proteins of hemoglobin (Hb), cytochrome C (Cyt C) and C-phycocyanin (C-PC) were chosen for the experiments.
Finally, the developed device was used for up to 8 h continuous separation of 5 mg/mL fuchsin acid and purification of three model proteins of phycocyanin, myoglobin and cytochrome C, demonstrating the applicability of FFE.
The availability of more reliable sequence search tools therefore helps to increase the success rates of the widely used approach to model proteins of unknown structures from homologous template structures [8].
To wit, mutations in the UPS can give rise to neurodegenerative disorders in both rodents and humans [ 11, 12] and sensitize organisms towards aggregation-prone model proteins of inherited neurological diseases [ 13, 14].
mCherry was used as a model protein to assess the efficiency of the constructed plasmid.
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