In the ACLT + MMx model, protein levels of MMP-13 were apparent in the differentiated chondrocytes and synovial cells of the cartilage surface at 2 weeks following surgery.
In one transient focal cerebral ischemia model, Parkin protein level was decreased further companied by increasing reperfusion time after 1 h left middle cerebral artery (MCA), while neuronal cell injury was increased companied by Parkin's reduction (Mengesdorf et al., 2002), which might be due to defective mitophagy.
With regard to peripheral nerve injury models, increased protein level of α2-δ-1 isoform in dorsal root ganglion (DRG) neurons on the ipsilateral side and its correlation with onset of allodynia have been reported.
They also report that prednisone treatment in the well-established mdx mouse model of DMD and the GRMD canine model increases protein levels of α7β1 integrin, as well as those of laminin-α2, which makes up a component of the basement membrane.
For the model analysis, protein levels were transformed by the natural logarithm then scaled.
It is also notable that, despite type I and III interferon responses being unchanged or reduced in our single-dose elastase + RV model, BAL protein levels of the interferon-stimulated gene CXCL10/IP-10 CXCL10/IP-10y enhanced.
On multivariate analysis using Cox proportional hazards model, low protein levels of p27 and high Ki-67 index were independent predictors of shortened disease-free (P< 0.0001, P = 0.0031, respectively), and low protein levels of p27, low staining of cyclin E and high Ki-67 index of overall survival (P = 0.0017, P = 0.0009, P = 0.0003, respectively).
Additionally, we assessed the influence of MAP kinase pathways on our inflammatory model at the protein level by immunohistochemistry. TG was treated as fresh, cultured or cultured in the presence of the specific MEK1/2 inhibitor U0126 [10−6 M].
A large number of catfish transcripts showed significant similarity with model fish at protein level as expected, suggesting that their function might also be conserved.
The straight line shows a linear fit with slope 1, that is, the best fit to a model where the protein level p is directly proportional to the mRNA level m, log(p ) = c + log(m ), with c = 7.06 (Appendix 1 ).
