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In this model protein expression was controlled by the neuron-specific enolase promoter and neither RT-PCR or Western blot analyses were able to detect expression of the prnp gene in non nervous tissues [5], [41].
In the lysophosphatidylcholine (LPC -induced focaLPC -inducedd demyelination mouse model, protein expression ofocalspinalincordsedemyelination
In our model, protein expression is instantaneous, producing a specified number of transposase dimers per transposon copy.
In order to model protein expression as a function of mRNA expression (disregarding for the moment all other factors that may influence protein expression), a decision has to be made regarding the form of the functional relationship.
With the large-scale quantification of proteins becoming possible, the integrative method can be modified to model protein expression alterations beyond mRNA expression alterations to reveal potential translational or post-translational regulators that lead to protein abundance changes during cancer progression.
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The multivariate model 2 is more appropriate for construction of a predictive model for protein expression, as it considers the combined effect of all miRNAs and thus weighs the contributions of the individual miRNAs to obtain optimal prediction.
SPIRE also connects results to publically available proteomics data through its Model Organism Protein Expression Database (MOPED).
Figure 2B shows the relative importance of each factor in the multivariate regression model for protein expression levels.
In agreement with this finding, in our experimental model PHD2 protein expression was upregulated in response to hypoxia at comparable levels in parental cells and bcl-2 overexpressing clones (data not shown).
We conclude that for the realistic parameter values used here, the NF model with protein expression variability is most consistent with experimental data.
The Model Organism Protein Expression Database (MOPED) is a proteomics repository that integrates protein expression information from MS-based proteomics experiments on human specimens and that from model organisms [7].
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