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Under guidance of the metabolic model prediction, a gapN-encoding NADP+ dependent glyceraldehyde-3-phosphate dehydrogenase pathway was constructed and then fine-tuned using five constitutive promoters.
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To commission the hot plate apparatus and validate the model predictions, a series of experiments were undertaken using sodium dithionite.
The data likelihoods were assumed to follow a lognormal distribution around the model predictions, a standard assumption with such measurements.
Since the R values were consistently larger for the expanded model predictions, a smaller percent increase is needed for the expanded model predictions than for the task-based predictions.
However, similar to model predictions, a clear antagonism was discovered between H3K36me3 and H3K27me3, both for the nucleation region and the gene body.
The model prediction shows a good agreement with experimental results.
The model prediction scored a success rate of 50.8%.
Results from experimental tensile testing of joints with different bonded areas were compared with model prediction showing a good correlation.
The model prediction provided a flood/contour map to visualize the synergetic gas-enrichment and higher-permeability regions.
Results from tensile tests performed in joints with different bonding areas are compared with model prediction showing a good agreement.
This suggests that the model prediction of a complex synergy between interacting signaling axes may be valid.
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