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Robert Martienssen, a pioneer in the study of epigenetics, investigates mechanisms involved in gene regulation and stem cell fate in yeast and model plants including Arabidopsis and maize.
Microarray technology has been applied to profile gene expression in response to abiotic stresses, such as drought, high salinity, cold, or ABA treatments in several model plants including Arabidopsis and rice (Kreps et al. 2002; Seki et al. 2002; Rabbani et al. 2003; Lenka et al. 2011).
It is noticed that rice may be a more suitable experimental plant model than other model plants including Arabidopsis, since it contains only one predicted UBC13 gene, while Arabidopsis contains two highly conserved and most likely redundant UBC13 genes (Wen et al. [2006]).
For this reason, maps have been generated from only a few model plants, including Arabidopsis and maize [ 13, 14].
Traditional cloning and sequencing methods have been used to identify miRNAs in model plants, including Arabidopsis, rice and poplar.
Identification of miRNAs has previously been reported in model plants, including in developing pollen of O. sativa[ 29] and mature pollen of Arabidopsis[ 30, 31] by using deep sequencing or miRNA arrays.
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When total genome sizes are compared for the model plants included in this analysis, the virtual transcripts of P. patens (511 Mb) represent 0.01% of genome size.
A codon-bias for the model plants included in this research (Chlamydomonas reinhardtii, Physcomitrella patens, Oryza sativa and Arabidopsis thaliana) was made comparing with the preferencial codon table for each species available at http://www.kazusa.or.jp/codon/.jp/codon/
The BESs from the minimal tiling path clones also provided insights into the genome composition of this novel model plant, including low GC content, transposable elements and gene content.
In particular, these Next-Generation Sequencing (NGS) methods provide a unique opportunity to advance studies of non-model plants, including economically important trees with complex genomes such as conifers [ 3- 5].
In the recent past, many De novo transcriptome data on non-model plants including Centella asiatica [ 13], Ramia [ 14], Liriodendron chinense [ 15], Gossypium aridum [ 16], and Gossypium hirsutum [ 17] have been generated by using the Illumina high throughput sequencing technology.
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