A third phylogenetic method was applied using the MrBayes program with mixed models [ 29]; where possible, model parameters were left to estimate by the program itself, and the best scoring tree out of 200,000 cycles from two runs with four Markov chains each was picked for comparison with the previous two methods.
(2004) did perform microarray experiments on a strain deleted for both the MSN2 and MSN4 transcription factors, they only performed two replicates with the double deletion strain, precluding statistical analysis of the data that would indicate its reliability for use in estimating model parameters, and leaving additional experiments to be performed.
Firstly, we consider chemical control only, with the other parameters left the same as in model (3.2).
In our model, the position parameter (left( beta right)) allows to switch between symmetric and asymmetric rules.
A discussion of the robustness of our quantizer to mismatches of the sensor model parameters is also left for Section 4. In this section, we discuss the computational complexity of the proposed algorithm by comparing with the previous work in[17].
After calculating the model parameters the leave-one-out cross validated R value was determined to be 0.616 with 3 latent variables, and the predicted versus measured survival values were plotted.
The model involves a total of six parameters, leaving their estimation as a typical over-parameterization problem.
The only parameter left in the model is the relative width σ̂, which we fit by using a least-squares method over a smoothed version of the empirical rank diversity.
The unknown parameter left in this spatial model is γ, the maximal production of Nodal.
The fitting was performed with a previously reported hybrid genetic algorithm with local search, leaving all model parameters free.
