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This explains why model organisms with decreased autophagy have accelerated aging, whereas enhanced stimulation may have potent anti-aging and disease-preventing effects14.
Here, we discuss the controversial question whether or not extant air-breathing fishes, such as mudskippers, amphibious gobioids that inhabit mangrove swamps, can be interpreted as living model organisms, with reference to the earliest land plants.
Therefore, genetically similar or even identical model organisms with disparate life expectancies should be much better suited for studying ageing.
Recent advances in technology, however, have produced a shift toward single nucleotide polymorphism (SNP) markers, particularly for model organisms with substantial genomic resources.
However, the absence of effective microscopic OPT implementations precluded the use of OPT in vivo, for imaging anatomical features of sub-millimeter-sized model organisms with adequate resolution.
As documented in earlier reports [28], [34], [41], creation of model organisms with targeted mutation of GRP94 allows comprehensive analysis of its in vivo function.
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Transcriptomic sequencing based on the next-generation sequencing technologies, provides a powerful and effective strategy to produce genome-scale sequence data, which allows the whole transcriptome to be investigated in a high-throughput and quantitative manner, especially for non-model organisms with genomic sequences that are yet to be measured22.
For non-model organisms with limited resources for manipulative studies, high-throughput transcriptomic data combined with bioinformatics methods provide a powerful approach to obtain initial insights into the function of unknown genes.
This is especially true for non-model organisms with comparatively few markers available and long generation time.
This strategy can be valuable for the cloning of mitogenomes from other non-model organisms with a sequenced transcriptome.
Luckily, RNA-Seq transcriptional profiling is a quite practical alternative to assess the functional genome of non-model organisms with no defined genome reference.
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