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While there are many tools available to design sgRNAs for the popular model organisms, only few tools that allow designing sgRNAs for non-model organisms exist.
Most of life sciences outside evolutionary biology, inclusive of phylogenetics and systematics, deal with a narrow selection of model organisms only, thus a paper in these disciplines will not often extend to comparisons between different organisms, one of which the author would be tempted to regard as lower or higher than others.
More importantly, given that in some model organisms only genetic screening data is available, our findings suggest that we can obtain substantial biological insights about genetically redundant pathways without inquiring the physical interaction data.
Consequently, compared to other model organisms, only relatively few sequenced ESTs and microarray cDNA targets are available for shrimp.
Model organisms only represent a small percentage of natural life, considering that numerous adaptation, morphological and behavioral traits are absent in model species.
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We do not invest time and resources into zebrafish as a model organism only because we are interested in zebrafish.
The yeast S. Pombe has been sequenced in 1999 and has been used as a model organism only relatively recently [23].
In the case of smaller cats (especially for the domestic cat as model organism) only 10 to 16 pieces per ovary are available.
Even for this well-studied model organism, only a small fraction of the transcriptional regulatory interactions are currently known [ 3, 5].
However, with mouse as a model organism, only a handful of lincRNA loci, when disrupted, have thus far resulted in overt phenotypes (Ponting and Belgard 2010).
Further, we address our hypothesis using amounts of sequence data that are typically available for many non-model organisms (only ~1.2 kb).
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