Step 7 was to reduce the list of ING-interacting proteins in the model organisms to just those satisfying two conditions: 1) strong pairwise ING-iteracting proteins homology between human, yeast and fly, and 2) having interaction data in both yeast and fly.
The systematic integration of the results obtained in a wide variety of high-throughput genetic screens in different model organisms has only just begun.
A key prerequisite for constructing a high-resolution linkage map is the availability of a large number of genetic markers (preferably, single-nucleotide polymorphisms SNPs), which has been unfortunately out of reach for most non-model organisms until just a few years ago due to the huge investments in large-scale marker discovery and array-based genotyping.
In vivo analysis using model organisms is also limited except just after fertilization during which microinjection and imaging are relatively easy.
Indeed, most drugs are developed and tested using model organisms; our ability to match not just sequence but also the activity of key genes in a specific disease would likely improve the process of drug discovery.
To counter these problems, we used intron data from sets of high-confidence gene annotations in three model organism species (taking just one isoform per locus).
Low frequency of DSB-independent HR limits this approach to just a few model organisms (e.g. Mus musculus, or Saccharomyces cerevisiae) and cell types (e.g. embryonic stem cells).
As an alternative, next generation sequencing is being proposed as a technology for EST development in non- model organisms, even if sequences produced are just short reads which can difficultly be properly assembled in the absence of a genomic sequence to provide transcriptome characterization [ 15- 17].
As taxon sampling has increased and Hox and ParaHox genes have been isolated from a wider range of species than just the traditional model organisms used in developmental biology, like Drosophila melanogaster and the mouse, it has become clear that there is a significant degree of evolutionary flexibility in the organization and function of the Hox and ParaHox genes.
But there are just a handful of model organisms, compared to the nearly nine million species on the earth and in the seas.
