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An ML phylogeny was derived for each Fot family, using a GTR + G (Gamma) model of substitutions.
Distance matrices were calculated with DNADIST program with Kimura's two-parameter model of substitutions and analyzed by Neighbor-joining tree-fitting algorithm with NEIGHBOR program.
M ODELTEST 3.06 [ 55] was used to choose the model of substitutions for the maximum-likelihood analysis of the combined data set.
For each of the 61 amino acid partitions, the most appropriate model of substitutions was determined by the BIC Bayesian Information Criterionn) in Modelgenerator ver. 0.43 [ 25].
Rose generates sets of related sequences based on a probabilistic model of substitutions and insertions/deletions for which the parameters can be adjusted by the user.
Each gene alignment was tested for the most appropriate model of substitutions using likelihood estimation and comparison with the Bayesian Information Criterion as implemented in the package "ape" [ 81] in R (The R Project - http://www.r-project.org/).org/
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Miyasaka's model of substitution solid solution is assumed for the crystalline complex.
The model of substitution was based on the suggestion by MODELTEST (http://darwin.uvigo.es/software/modeltest.html).html
Amino-acid analysis was based on the POISSON model and nucleotide analysis adopted a codon-based GTR+G+I model of substitution.
Maximum likelihood (ML) analyses on the moth and plant matrices were performed under the GTR + G model of substitution using RAxML [34].
Coding sequences were partitioned by codon and the analyses were run using a GTR+I+γ model of substitution selected using jModel test [66].
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