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We examined the roles of IL-1β and TNF-α in basement membrane formation in an in vitro model of alveolar epithelial tissue composed of alveolar epithelial cells and pulmonary fibroblasts.
We aimed to investigate whether EPCs can enhance the osteogenic properties of MSCs in vitro, and whether transplantation of EPC-MSC cell sheets could promote bone regeneration in a rat model of alveolar bone defect.
In this pig model of alveolar edema, an AFC rate of around 20% in the first hour was observed at both edema levels studied (4 ml/kg and 10 ml/kg).
Tsuchida et al. demonstrated that in a model of alveolar collapse and surfactant depletion the atelectasis regions per se were protected from alveolar damage, whereas alveoli from the aerated regions were more affected [ 15].
Implications and future directions In summary, these data validate the ATII-to-ATI cell trans-differentiation in vitro system as a suitable model of alveolar epithelial cell injury and wound repair in vivo.
More than three decades ago, Mead and coworkers [ 7] described a simplified model of alveolar mechanics in which they proposed that an alveolus attempting to close in an environment in which it was surrounded by inflated tissue would experience traction forces that are amplified in nonlinear proportion to the alveolar pressures existing in the open units.
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Recent studies have focused on dentin as a potential bone substitute in different models of alveolar defects.
Cell culture models of alveolar epithelia, either primary culture or immortalized cell line, have advantages over whole organ models including controllable conditions, repeatable injuries and treatments, lower costs, and high study throughput.
In summary, the beneficial effects of RMs have been demonstrated in animal models of alveolar collapse induced by surfactant depletion.
As an alternative, simpler in vitro models of alveolar epithelium have been widely used to study important biological and functional characteristics.
Our results also suggest that monocyte-derived MΦ can be used as a PRRSV infection model instead of alveolar MΦ, avoiding the killing of pigs.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com