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Previous experiments in an HT-29 tumor model grown in NOD-SCID mice have shown that the 10 mg/kg nal-IRI dose achieves a prolonged SN-38 tumor duration of ~96 h, while the exposure with 50 mg/kg free irinotecan is ~36 h [24].
UCP2 content, measured by calibrated immunoblot in INS-1E insulinoma cells (a pancreatic beta-cell model) grown in RPMI medium, and INS-1E mitochondria, was 2.0 ng/million cells (7.9 ng/mg mitochondrial protein).
Because the ultimate goal was to examine the efficacy of VEGF antisense treatment in a human tumour model grown in a mouse host, we evaluated the effect on both human (HMVEC-L) and mouse (MHE) endothelial cells.
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CEO of Professional Science Editing for Scientists @ prosciediting.com