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This work offered a perfect starting point to construct a kinetic model for fatty acid metabolism with particular focus on the β-oxidation cycle and the objective to enhance our understanding of various deficiencies of acyl-CoA dehydrogenases.
To verify the in vivo results, a human hepatocellular cell line, SK-HEP-1 cells, was challenged with 1 mM oleic acid [ 10] for 48 h to establish a cellular model for fatty liver disease.
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39 However, due to space limitations we will not discuss the different models for fatty acid transport in details, but rather refer to several comprehensive reviews which cover this topic.
We manually annotated and summarized the gene models for fatty acid and TAG biosynthesis that were predicted in this work, together with related data that have been deposited to GenBank (Supplementary Table S7).
Recent studies of transgenic and knockout animal models for fatty acid transporters and the identification of fatty acid transport protein 4 (FATP4 or SLC27A4) mutations as causative for Ichthyosis Prematurity Syndrome highlight the vital roles of fatty acid transport and metabolism in skin homeostasis.
The SCP-2/membrane collision model is the proposed mode for fatty acid transport by the SCP-2 of Yarrowia lipolytica [ 43].
We report for the first time the liquid phase heterogeneous oxidation of 1-tetradecanol, used as a model molecule for fatty alcohols, according to green chemistry principles by using a Au/CeO2 Al2O3 catalyst and O2 as oxidant at normal pressure.
These findings suggest a revised model for the fatty acid synthase in which the two polypeptides are oriented such that head-to-tail contacts are formed both between and within subunits.
Genetically obese, leptin-deficient ob/ob mice, a model for nonalcoholic fatty liver disease, and their lean litter mates were treated with a small dose of LPS.
These results therefore suggest that Dictyostelium could provide a suitable model for liver fatty acid uptake and steatosis research, and will enable a combined genetic and biochemical approach to be used in the future to better understand this effect.
Finally, a predictive, theoretical model of fatty acid binding was developed that accounted for the observed "overshoot" kinetics.
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