Besides, cellular changes in osteoblast morphology and orientation using this model of fluid shear stress were proven.
Bioreactor designs for bone tissue engineering applications often use perfusion models where fluid shear stresses through porous scaffolds activate biological processes such as cell proliferation and differentiation [ 1– 5].
In our early studies, Salmonella enterica serovar Typhimurium (hereafter referred to as S. typhimurium or Salmonella) was cultured in a ground-based bioreactor that was used to model the low fluid shear culture conditions that occur in microgravity as well as in certain in vivo environments.
The finite element model agreed with classical analytical models for pressure drop, fluid shear, and concentration polarization in straight-through open channels at low flux and high cross flow.
The lectin-mediated cell adhesion further showed an enhanced resistance to trypsin (as a protease model) and to applied fluid shear stress (mechanical stability).
When calculating the forces on the sphere due to fluid shear, their model uses the Goldman equation [ 27]: the amount of force a sphere experiences from an applied shear force is calculated by assuming that the sphere is solid.
Additionally, in an in vitro fluid shear flow model, exogenously added sL-selectin inhibited leukocyte rolling and firm adhesion to the endothelium in a dose-dependent manner, presumably by competing with cell surface L-selectin for binding of endothelial ligands [ 23].
In this study, we computed the fluid shear stresses in model annular vessels over a range of laminar flow regimes, from Couette flow to Taylor-vortex flow, and at two geometric scales, using a shear rate model for freely suspended particles, together with experimental Laser Doppler Anemometry data for a 2-D velocity field.
Experimentally, chemotaxis can generate neovascularization [ 72], and for the current model, we neglected mechanical factors, including fluid shear stress and explicit changes in matrix stiffness.
This is confirmed from our model results which imply that the fluid shear stress in the cell layer is dependent on both the upper fluid inlet flux and cell layer width, both of which are varied as part of our investigation.
