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By using this regression model, each sequence-predicted miRNA-mRNA interaction is assigned one coefficient; this coefficient represents how much the inferred activity profile of that miRNA contributes to predicting that mRNA's "down-regulation" profile (see Methods) when considering the activity profiles of all other miRNAs predicted to target the mRNA.
Moreover, for the different models, each sequence contains different amount of motif instances, so the value of λ f can be set by different models and Pocc.
The database contains action configurations, such as positions of body parts, pose descriptors from silhouette images, a stochastic model encoding each sequence of the pose descriptors, and a regression model for predicting the configuration from the pose descriptor.
This step requires fitting an individual HMM model for each sequence in the training data (mathfrak {O_{l}}_{textit {Trn}}^{Feat}).
MrModeltest [ 52, 53] was used to select the best-fitting evolutionary model for each sequence region.
Plots of the two- vs one-state model for each sequence are shown in the Supporting Information.
We also used MEGA 6.0 to perform statistical selection of the nucleotide substitution model for each sequence collection.
When the model underlying each sequence is known, then it is possible to display ROC curves using the WISCOD exon testing module.
For the remaining 32,488 reference sequences, we fit a separate generalized linear model to each sequence, with count as the dependent variable and treatment group as the independent variable.
The best substitution model for each sequence was chosen based on analysis in MEGA 5.0 (K2 + G for the small tree of 16S rRNA; GTR + G + I for mcyD concatenated with mcyE; K2 + G + I for long tree of 16S rRNA; JTT + G + I for condensation, adenylation, and epimerization domains sequences of McyA; and JTT + G for peptidyl carrier protein domain sequences of McyA).
The Akaike Information Criterion in MrModelTest v2.3 [ 53] was used to choose the best-fitting evolutionary model for each sequence region (GTR+Γ+I for both partitions in the Orchidaceae data set and GTR+Γ for ITS in the Hoffmannseggella data set).
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