The Discovery Studio package (v1.7, Accelrys, San Diego, CA) was used for model construction and refinement.
The 2D image dataset was increased to include all of the particles and iterative refinement continued.
Kosinski, J. et al. A 'FRankenstein's monster' approach to comparative modeling: merging the finest fragments of Fold-Recognition models and iterative model refinement aided by 3D structure evaluation.
Iterative runs of ARP/wARP combined with manual model building and iterative cycles of refinement in REFMAC were used to complete and refine the model at 1.55 Å resolution.
The technique uses abstraction based over-approximations of the model behaviour, combined with iterative refinement.
Our approach employs systematic knowledge elicitation and practically feasible evaluation techniques performed as part of the modelling process for iterative refinement of the models.
CycSim facilitates the identification of inconsistencies, the design of new experiments and the iterative refinement of models using experimental data.
Process to be followed, e.g., modified waterfall model, iterative refinement, incremental development, phased development, etc. Outline plan, showing principal activities and milestones.
Qualitative simulation would facilitate the iterative refinement of whole-cell mechanistic models through both theoretical prediction and experimental validation.
Further, future efforts to develop predictive models of anopheline cell signaling responses, based on iterative construction and refinement of data-based and literature-based knowledge of the MAP kinase cascades and other networked pathways (reviewed in [ 65]), will ultimately be necessary to elucidate the "master signaling regulators" in these biomedically important insects.
Further, future efforts to develop predictive models of anopheline cell signaling responses, based on iterative construction and refinement of data-based and literature-based knowledge of the MAP kinase cascades and other networked pathways will facilitate identification of the "master signaling regulators" in biomedically important mosquito species.
