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The substitution model, clock model and tree model of the two genes were set as unlinked.
We estimated dS, dN, and dN/ dS for the protein-coding sequences in CODEML in PAML (version 4.4b, Yang 2007), using the F3x4 codon frequency model (clock = 0).
Non-consumables such as flip-charts, uniforms for implementers, and a model clock were produced just once for use throughout the intervention.
Divergence times were estimated under a relaxed clock model in the MCMCTree program in the PAML4.7 package [ 28], with "Independent rates model (clock = 2)" and "JC69 model" selected for our calculations.
The relaxed model clock following a lognormal distribution was also supported by the highest log10 BF as suggested [ 39]. aMean prior and marginal posterior probabilities for both clock models are given with 95% lower and upper bounds of the highest probability density intervals in parentheses.
The IR model has been considered more appropriate in divergence time estimation than the autocorrelated-rates (AR) model in recent studies (see [ 66] and references therein), although additional analyses using AR model (clock = 3 in MCMCTREE) were also performed for comparison.
Similar(54)
Loci were allowed unlinked substitution models, clock models and partition trees.
To that extent, a likelihood ratio test was performed between the null model (global clock) and alternative model (local clock).
In addition to the substitution model, the clock model and tree topologies were estimated independently for each gene.
Thus, each locus had its own model and clock rate specified.
The mechanical models of clock mechanisms are of some explanatory help.
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