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To determine the specific components on the cell surface, Palmitoyloleoylphosphatidylcholine/dioleoylphosphatidylserine (POPC/DOPS) - supported bilayer was used as a model cell membrane and its TERS spectra were correlated with the near-field spectra from human dermal derived keratinocyte (HaCaT) cells.
In this study, we examine mechanical disruption of a model cell membrane in the presence of a representative magnetic nanoparticle coating, the copolymer poly ethylene oxide)–poly ethyl ethylene) (PEO PEE).
We first established the specificity of HA binding to glycan receptors on the model cell membrane.
They have been widely used as a model cell membrane system and as a cell-sized container [ 11, 80].
As a first step toward understanding the mechanism by which the GBP-glycan complexes are formed, we have investigated the binding kinetics of recombinant HA from the H5N1 avian influenza A virus to specific glycan receptors on model cell membrane surfaces.
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Compared to parent GO, intermediate photoproducts exhibited reduced interaction with model cell membranes and altered biomarker responses.
Model cell membranes such as substrate supported lipid bilayers and lipid vesicles were used in this research.
Such techniques are widely used today in many branches of physics and engineering, by insurers calculating risk, and even by computational biologists to model cell membranes or proteins.
It is essential to evaluate the interactions of nanoscale materials with model cell membranes to gain insight into the chemical, physical, and biological effects of nanoscale materials on living cells.
Small unilamellar vesicles used as model cell membranes were prepared from 1,2-dipalmitoyl-3-sn-phosphatidylcholine (DPPC), and mixed with varying concentrations of organic salts and classic protective compounds.
Required functionalization steps include: (1) engineering of the semiconductor surfaces with organic molecules, (2) deposition of biocompatible polymer films and control of their interfacial characteristics, (3) physical modeling of cell surface glycocalix, (4) reconstitution and characterization of native- and model cell membranes.
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