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We chose this metric, as we use the time-lagged response and design matrices for model building (see Section 2).

Nonetheless, stepwise model building (see " Methods") indicated that MSP assay classification could be predicted even better using two or more probes.

All causal connections or edges were based on manually curated literature (for details of network model building, see Refs. 25– 25).

For a more technical discussion of the algorithms used in defining these measures and for a more detailed description on model building, see the Overview, Design Concepts, and Details (ODD) available as an Additional file 1, which follows the protocol for describing agent-based models as suggested by Grimm et al. [ 43, 49].

The resulting equation predicts the FGF - FD combinations the anesthesiologist can use to attain a FAt within the same time interval used during model development (i.e., between 1 and 5 min) using a single FD and FGF setting, and takes into account the covariate effects derived during model building (see results section for actual equation).

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However, both variables were kept in the final multivariable models because of their significant effects in respective flexible spline-based models, estimated at the step 2 of the model building process (see 'Statistical analyses').

The training data was further randomly divided 2 1 into a training set used for model building (1831 molecules, see additional file 2: internaltraining.csv) and an internal test set (915 molecules, see additional file 3: internaltest.csv).

Extensive model building and testing (see Supporting Information) revealed that soaking with Neu5Ac in the Y137A variant serendipitously produced a structure of the Enz-ManNAc-pyruvate complex in subunit C.

In summary, considering the profile-profile comparisons E-values, secondary structure predictions, fold assignment detection and the results of the homology model building and evaluation (see Additional file 1), we are confident that this domain is a new member of the Ubq superfamily.

The entire building seen in the exhibition in a model — would be enclosed by a Fuller-designed geodesic dome.

Both S3 and S4 could move relatively independently from S1 and S2, owing to a 20-amino acid long loop sequence residing between the bottom parts of S2 and S3 (see model building in Methods).

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